A platform for evaluating sperm RNA biomarkers: Dysplasia of the fibrous sheath - Testing the concept

Aletheia Lima-Souza, Ester Anton, Shihong Mao, Won Jin Ho, Stephen A. Krawetz

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Objective: To investigate the potential use of correlative microarray-based transcript pairs as candidate markers for male fertility using dysplasia of the fibrous sheath (DFS) as an affected model. It is widely recognized that microarray technology may be limited by cost and that the quality of the transcript remains relatively unknown. To address these issues, we analyzed the stable transcript pairs by qPCR with a systematic primer design process. Design: Experimental study. Setting: University. Patient(s): Men with proven fertility and men with a diagnosis of DFS. Intervention(s): None. Main Outcome Measure(s): Primer sequences for six genes of interest were designed using Oligo7 and Primer3Plus. Primer specificity was initially assessed in silico by searching the ENSEMBL, University of California Santa Cruz, and National Center for Biotechnology Information databases for nontarget complementary sequences throughout the genome. The ability of transcript pairs to classify samples from males of proven fertility away from DFS was assessed. Result(s): In conjunction with identifying four new stable transcript pairs, comparison of the DFS qPCR C(t) correlation coefficients revealed the disruption of four stable fertile sample transcript pairs. This suite of transcript pairs resolves DFS. Conclusion(s): The results show that with effectively designed primers, qPCR may provide an affordable molecular assay to assess male fertility status. © 2012 American Society for Reproductive Medicine, Published by Elsevier Inc.
Original languageEnglish
JournalFertility and Sterility
Volume97
Issue number5
DOIs
Publication statusPublished - 1 Jan 2012

Keywords

  • Biomarker
  • male infertility
  • primer design
  • real-time PCR

Fingerprint Dive into the research topics of 'A platform for evaluating sperm RNA biomarkers: Dysplasia of the fibrous sheath - Testing the concept'. Together they form a unique fingerprint.

  • Cite this