A novel bio-functional material based on mammalian cell aggresomes

Escarlata Rodríguez-Carmona, Rosa Mendoza, Eugènia Ruiz-Cánovas, Neus Ferrer-Miralles, Ibane Abasolo, Simó Schwartz, Antonio Villaverde, José Luis Corchero

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

© 2015, Springer-Verlag Berlin Heidelberg. Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the physiological roles of these protein clusters and the functional status of the embedded proteins remain basically unexplored. In this work, we have determined for the first time that, like in bacterial inclusion bodies, deposition of recombinant proteins into aggresomes does not imply functional inactivation. As a model, human α-galactosidase A (GLA) has been expressed in mammalian cells as enzymatically active, mechanically stable aggresomes showing higher thermal stability than the soluble GLA version. Since aggresomes are easily produced and purified, we propose these particles as novel functional biomaterials with potential as carrier-free, self-immobilized catalyzers in biotechnology and biomedicine.
Original languageEnglish
Pages (from-to)7079-7088
JournalApplied Microbiology and Biotechnology
Volume99
DOIs
Publication statusPublished - 18 Sep 2015

Keywords

  • Aggresomes
  • Immobilized catalyzer
  • Protein nanoparticles
  • Recombinant protein

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