A novel application of immobilized enzymes: Affinity chromatography separations using enzymes depending of a cofactor

J. M. Serrat; G. Caminal; F. Gòdia; C. Solà; J. López-Santín

doi:https://doi.org/10.1007/BF02447487

A novel application of immobilized enzymes: Affinity chromatography separations using enzymes depending of a cofactor

J. M. Serrat, G. Caminal, F. Gòdia, C. Solà, J. López-Santín

Department of Chemistry

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Abstract

A novel application of immobilized enzymes is presented. Cofactor dependent immobilized enzymes can be used as affinity chromatography supports to bind their substrates without alteration in absence of the cofactor. The procedure has been demonstrated using β-galactose dehydrogenase to retain D-galactose selectively in the absence of NAD. The uses of the technique are discussed from the down-stream processing and mechanistic points of view. © 1992 Science & Technology Letters.

Original language	English
Pages (from-to)	451-454
Journal	Biotechnology Techniques
Volume	6
DOIs	https://doi.org/10.1007/BF02447487
Publication status	Published - 1 Sept 1992

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title = "A novel application of immobilized enzymes: Affinity chromatography separations using enzymes depending of a cofactor",

abstract = "A novel application of immobilized enzymes is presented. Cofactor dependent immobilized enzymes can be used as affinity chromatography supports to bind their substrates without alteration in absence of the cofactor. The procedure has been demonstrated using β-galactose dehydrogenase to retain D-galactose selectively in the absence of NAD. The uses of the technique are discussed from the down-stream processing and mechanistic points of view. {\textcopyright} 1992 Science & Technology Letters.",

author = "Serrat, {J. M.} and G. Caminal and F. G{\`o}dia and C. Sol{\`a} and J. L{\'o}pez-Sant{\'i}n",

year = "1992",

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language = "English",

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AU - Serrat, J. M.

AU - Caminal, G.

AU - Gòdia, F.

AU - Solà, C.

AU - López-Santín, J.

PY - 1992/9/1

Y1 - 1992/9/1

N2 - A novel application of immobilized enzymes is presented. Cofactor dependent immobilized enzymes can be used as affinity chromatography supports to bind their substrates without alteration in absence of the cofactor. The procedure has been demonstrated using β-galactose dehydrogenase to retain D-galactose selectively in the absence of NAD. The uses of the technique are discussed from the down-stream processing and mechanistic points of view. © 1992 Science & Technology Letters.

AB - A novel application of immobilized enzymes is presented. Cofactor dependent immobilized enzymes can be used as affinity chromatography supports to bind their substrates without alteration in absence of the cofactor. The procedure has been demonstrated using β-galactose dehydrogenase to retain D-galactose selectively in the absence of NAD. The uses of the technique are discussed from the down-stream processing and mechanistic points of view. © 1992 Science & Technology Letters.

U2 - https://doi.org/10.1007/BF02447487

DO - https://doi.org/10.1007/BF02447487

M3 - Article

VL - 6

SP - 451

EP - 454

ER -

A novel application of immobilized enzymes: Affinity chromatography separations using enzymes depending of a cofactor

Abstract

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