A novel application of immobilized enzymes: Affinity chromatography separations using enzymes depending of a cofactor

J. M. Serrat, G. Caminal, F. Gòdia, C. Solà, J. López-Santín

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1 Citation (Scopus)

Abstract

A novel application of immobilized enzymes is presented. Cofactor dependent immobilized enzymes can be used as affinity chromatography supports to bind their substrates without alteration in absence of the cofactor. The procedure has been demonstrated using β-galactose dehydrogenase to retain D-galactose selectively in the absence of NAD. The uses of the technique are discussed from the down-stream processing and mechanistic points of view. © 1992 Science & Technology Letters.
Original languageEnglish
Pages (from-to)451-454
JournalBiotechnology Techniques
Volume6
DOIs
Publication statusPublished - 1 Sep 1992

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