A new automated turbidimetric immunoassay for the measurement of canine C-reactive protein

Matilde Piñeiro, Raquel Pato, Lourdes Soler, Raquel Peña, Natalia García, Carlos Torrente, Yolanda Saco, Fermín Lampreave, Anna Bassols, Francesca Canalias

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

© 2018 American Society for Veterinary Clinical Pathology Background: In dogs, as in humans, C-reactive protein (CRP) is a major acute phase protein that is rapidly and prominently increased after exposure to inflammatory stimuli. CRP measurements are used in the diagnosis and monitoring of infectious and inflammatory diseases. Objectives: The study aim was to develop and validate a turbidimetric immunoassay for the quantification of canine CRP (cCRP), using canine-specific reagents and standards. Methods: A particle-enhanced turbidimetric immunoassay was developed. The assay was set up in a fully automated analyzer, and studies of imprecision, limits of linearity, limits of detection, prozone effects, and interferences were carried out. The new method was compared with 2 other commercially available automated immunoassays for cCRP: one turbidimetric immunoassay (Gentian CRP) and one point-of-care assay based on magnetic permeability (Life Assays CRP). Results: The within-run and between-day imprecision were <1.7% and 4.2%, respectively. The assay quantified CRP proportionally in an analytic range up to 150 mg/L, with a prozone effect appearing at cCRP concentrations >320 mg/L. No interference from hemoglobin (20 g/L), triglycerides (10 g/L), or bilirubin (150 mg/L) was detected. Good agreement was observed between the results obtained with the new method and the Gentian cCRP turbidimetric immunoassay. Conclusions: The new turbidimetric immunoassay (Turbovet canine CRP, Acuvet Biotech) is a rapid, robust, precise, and accurate method for the quantification of cCRP. The method can be easily set up in automated analyzers, providing a suitable tool for routine clinical use.
Original languageEnglish
Pages (from-to)130-137
JournalVeterinary Clinical Pathology
Volume47
Issue number1
DOIs
Publication statusPublished - 1 Mar 2018

Keywords

  • Acute phase proteins
  • C-reactive protein
  • Method comparison

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