A multiple path photonic lab on a chip for parallel protein concentration measurements

Isaac Rodríguez-Ruiz, Mayte Conejero-Muriel, Tobias N. Ackermann, José A. Gavira, Andreu Llobera

    Research output: Contribution to journalArticleResearchpeer-review

    10 Citations (Scopus)

    Abstract

    © The Royal Society of Chemistry 2015. We propose a PDMS-based photonic system for the accurate measurement of protein concentration with minute amounts of the sample. As opposed to the state of the art approach, in the multiple path photonic lab on a chip (MPHIL), analyte concentration or molar absorptivity is obtained with a single injection step, by performing simultaneous parallel optical measurements varying the optical path length. Also, as opposed to the standard calibration protocol, the MPHIL approach does not require a series of measurements at different concentrations. MPHIL has three main advantages: firstly the possibility of dynamically selecting the path length, always working in the absorbance vs. concentration linear range for each target analyte. Secondly, a dramatic reduction of the total volume of the sample required to obtain statistically reliable results. Thirdly, since only one injection is required, the measurement time is minimized, reducing both contamination and signal drifts. These characteristics are clearly advantageous when compared to commercial micro-spectrophotometers. The MPHIL concept was validated by testing three commercial proteins, lysozyme (HEWL), glucose isomerase (d-xylose-ketol-isomerase, GI) and Aspergillus sp. lipase L (BLL), as well as two proteins expressed and purified for this study, B. cereus formamidase (FASE) and dihydropyrimidinase from S. meliloti CECT41 (DHP). The use of MPHIL is also proposed for any spectrophotometric measurement in the UV-VIS range, as well as for its integration as a concentration measurement platform in more advanced photonic lab on a chip systems. This journal is
    Original languageEnglish
    Pages (from-to)1133-1139
    JournalLab on a Chip
    Volume15
    Issue number4
    DOIs
    Publication statusPublished - 21 Feb 2015

    Fingerprint

    Dive into the research topics of 'A multiple path photonic lab on a chip for parallel protein concentration measurements'. Together they form a unique fingerprint.

    Cite this