A method for the study of the spatial distribution of the neuronal dendritic tree using a universal stage

Pere J. Berbel, Juan J. Villanueva, Jose Regidor, Carlos Lopez-Garcia

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    3 Citations (Scopus)

    Abstract

    The use of a microscope equipped with a universal stage with 4 rotation axes, drawing tube and photographic system for the 3-dimensional study of neuronal morphology is considered. Two-dimensional projections of the neuronal tree are obtained by rotating the stage, and the application of coordinate transformations results in a 3-dimensional mapping of neuronal topography. Algorithms used for these transformations are presented and the method applied to Golgi-impregnated neurons of the cerebral cortex of adult lizards. The advantages, limitations and sources of error of this method are discussed. © 1981.
    Original languageEnglish
    Pages (from-to)141-152
    JournalJournal of Neuroscience Methods
    Volume4
    Issue number2
    DOIs
    Publication statusPublished - 1 Jan 1981

    Keywords

    • 3-dimensional neuronal morphology
    • Golgi-impregnated neurons
    • neuronal rotation
    • universal stage

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