A controlled fed-batch cultivation for the production of new crude lipases from Candida rugosa with improved properties in fine chemistry

A. Sánchez, P. Ferrer, A. Serrano, F. Valero, C. Solà, M. Pernas, M. L. Rúa, R. Fernández-Lafuente, J. M. Guisán, R. M. De La Casa, J. V. Sinisterra, J. M. Sánchez-Montero

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    Abstract

    A controlled constant feeding rate fed-batch strategy using oleic acid as inducer produced a crude lipase preparation from Candida rugosa (CRL-UAB) with higher protein content, carbohydrate content and lipase activity than commercial Sigma type VII CRL. CRL-UAB was partially characterised and tested in selective biotransformations of chiral compounds in aqueous (2-hydroxy 4-phenyl butanoic acid ethyl ester (HPBE)) and organic media (2-phenyl propionic acid and ketoprofen). CRL-UAB showed higher substrate specificity and enantioselectivity in aqueous media compared to Sigma CRL. Also, higher specific initial rates with 2-phenyl propionic acid and ketoprofen were observed in organic media. The influence of water on the esterification of ketoprofen was not relevant with CRL-UAB under the conditions tested, whereas a dramatic influence was observed in Sigma CRL. Different CRL-UAB batches obtained under the same cultivation controlled conditions were identical from the point of view of chromatographic behaviour, immobilisation rates and catalytic properties, indicating that a reproducible C. rugosa lipase extract had been obtained. Copyright (C) 1999 Elsevier Science B.V.
    Original languageEnglish
    Pages (from-to)169-182
    JournalJournal of Biotechnology
    Volume69
    DOIs
    Publication statusPublished - 15 Apr 1999

    Keywords

    • Candida rugosa
    • Characterisation
    • Esterification of 2-aryl propionic derivatives
    • Hydrolysis of HPBE
    • Immobilisation
    • Lipase
    • Production

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    Sánchez, A., Ferrer, P., Serrano, A., Valero, F., Solà, C., Pernas, M., Rúa, M. L., Fernández-Lafuente, R., Guisán, J. M., De La Casa, R. M., Sinisterra, J. V., & Sánchez-Montero, J. M. (1999). A controlled fed-batch cultivation for the production of new crude lipases from Candida rugosa with improved properties in fine chemistry. Journal of Biotechnology, 69, 169-182. https://doi.org/10.1016/S0168-1656(99)00045-0