TY - JOUR
T1 - A comprehensive study of the harmful effects of ZnO nanoparticles using Drosophila melanogaster as an in vivo model
AU - Alaraby, Mohamed
AU - Annangi, Balasubramanyam
AU - Hernández, Alba
AU - Creus, Amadeu
AU - Marcos, Ricard
PY - 2015/10/5
Y1 - 2015/10/5
N2 - © 2015 Elsevier B.V. This study planned to determine the range of biological effects associated with ZnO-NP exposure using Drosophila melanogaster as an in vivo model. In addition, ZnCl2 was used to determine the potential role of Zn ions alone. Toxicity, internalization through the intestinal barrier, gene expression changes, ROS production, and genotoxicity were the end-points evaluated. No toxicity or oxidative stress induction was observed in D. melanogaster larvae, whether using ZnO-NPs or ZnCl2. Internalization of ZnO-NPs through the intestinal barrier was observed. No significant changes in the frequency of mutant clones (wing-spot test) or percentage of DNA in tail (comet assay) were observed although significant changes in Hsp70 and p53 gene expression were detected. Our study shows that ZnO-NPs do not induce toxicity or genotoxicity in D. melanogaster, although uptake occurs and altered gene expression is observed.
AB - © 2015 Elsevier B.V. This study planned to determine the range of biological effects associated with ZnO-NP exposure using Drosophila melanogaster as an in vivo model. In addition, ZnCl2 was used to determine the potential role of Zn ions alone. Toxicity, internalization through the intestinal barrier, gene expression changes, ROS production, and genotoxicity were the end-points evaluated. No toxicity or oxidative stress induction was observed in D. melanogaster larvae, whether using ZnO-NPs or ZnCl2. Internalization of ZnO-NPs through the intestinal barrier was observed. No significant changes in the frequency of mutant clones (wing-spot test) or percentage of DNA in tail (comet assay) were observed although significant changes in Hsp70 and p53 gene expression were detected. Our study shows that ZnO-NPs do not induce toxicity or genotoxicity in D. melanogaster, although uptake occurs and altered gene expression is observed.
KW - Comet assay
KW - Drosophila melanogaster
KW - Genotoxicity
KW - Wing-spot assay
KW - ZnO nanoparticles
U2 - https://doi.org/10.1016/j.jhazmat.2015.04.053
DO - https://doi.org/10.1016/j.jhazmat.2015.04.053
M3 - Article
SN - 0304-3894
VL - 296
SP - 166
EP - 174
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
ER -