A comprehensive study of the harmful effects of ZnO nanoparticles using Drosophila melanogaster as an in vivo model

Mohamed Alaraby, Balasubramanyam Annangi, Alba Hernández, Amadeu Creus, Ricard Marcos

Research output: Contribution to journalArticleResearchpeer-review

35 Citations (Scopus)

Abstract

© 2015 Elsevier B.V. This study planned to determine the range of biological effects associated with ZnO-NP exposure using Drosophila melanogaster as an in vivo model. In addition, ZnCl<inf>2</inf> was used to determine the potential role of Zn ions alone. Toxicity, internalization through the intestinal barrier, gene expression changes, ROS production, and genotoxicity were the end-points evaluated. No toxicity or oxidative stress induction was observed in D. melanogaster larvae, whether using ZnO-NPs or ZnCl<inf>2</inf>. Internalization of ZnO-NPs through the intestinal barrier was observed. No significant changes in the frequency of mutant clones (wing-spot test) or percentage of DNA in tail (comet assay) were observed although significant changes in Hsp70 and p53 gene expression were detected. Our study shows that ZnO-NPs do not induce toxicity or genotoxicity in D. melanogaster, although uptake occurs and altered gene expression is observed.
Original languageEnglish
Pages (from-to)166-174
JournalJournal of Hazardous Materials
Volume296
DOIs
Publication statusPublished - 5 Oct 2015

Keywords

  • Comet assay
  • Drosophila melanogaster
  • Genotoxicity
  • Wing-spot assay
  • ZnO nanoparticles

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