Abstract
Mycoplasma genitalium is a human pathogen associated with several sexually transmitted diseases. Proteomic technologies, along with other methods for global gene expression analysis, play a key role in understanding the mechanisms of bacterial pathogenesis and physiology. The proteome of M. genitalium, model of a minimal cell, has been extended using a combination of different proteomic approaches and technologies. The total proteome of this microorganism has been analyzed using gel-based and gel-free approaches, achieving the identification of 85.3% of the predicted ORFs. In addition, a comprehensive analysis of membrane subproteome has been performed. For this purpose, the TX-114 soluble fraction has been analyzed as well as the surface proteins, using cell-surface protein labeling with CyDye. Finally, the serological response of M. genitalium-infected patients and healthy donors has been analyzed to identify proteins that trigger immunological response. Here, we present the most extensive M. genitalium proteome analysis (85.3% of predicted ORFs), a comprehensive M. genitalium membrane analysis, and a study of the human serological response to M. genitalium. © 2012 American Chemical Society.
| Original language | English |
|---|---|
| Pages (from-to) | 3305-3316 |
| Journal | Journal of Proteome Research |
| Volume | 11 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Jun 2012 |
Keywords
- immunoproteome
- membrane proteome
- Mycoplasma genitalium
- surfome
- total proteome
