3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies

Laura Saenz Del Burgo, Jesús Ciriza, Albert Espona-Noguera, Xavi Illa, Enric Cabruja, Gorka Orive, Rosa María Hernández, Rosa Villa, Jose Luis Pedraz, Mar Alvarez

    Research output: Contribution to journalArticleResearch

    8 Citations (Scopus)

    Abstract

    © 2018 The Author(s). Cell microencapsulation is an attractive strategy for cell-based therapies that allows the implantation of genetically engineered cells and the continuous delivery of de novo produced therapeutic products. However, the establishment of a way to retrieve the implanted encapsulated cells in case the treatment needs to be halted or when cells need to be renewed is still a big challenge. The combination of micro and macroencapsulation approaches could provide the requirements to achieve a proper immunoisolation, while maintaining the cells localized into the body. We present the development and characterization of a porous implantable macrocapsule device for the loading of microencapsulated cells. The device was fabricated in polyamide by selective laser sintering (SLS), with controlled porosity defined by the design and the sintering conditions. Two types of microencapsulated cells were tested in order to evaluate the suitability of this device; erythropoietin (EPO) producing C2C12 myoblasts and Vascular Endothelial Growth Factor (VEGF) producing BHK fibroblasts. Results showed that, even if the metabolic activity of these cells decreased over time, the levels of therapeutic protein that were produced and, importantly, released to the media were stable.
    Original languageEnglish
    Article number8512
    JournalScientific Reports
    Volume8
    DOIs
    Publication statusPublished - 1 Dec 2018

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    Saenz Del Burgo, L., Ciriza, J., Espona-Noguera, A., Illa, X., Cabruja, E., Orive, G., Hernández, R. M., Villa, R., Pedraz, J. L., & Alvarez, M. (2018). 3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies. Scientific Reports, 8, [8512]. https://doi.org/10.1038/s41598-018-26869-5