γH2AX foci on apparently intact mitotic chromosomes: Not signatures of misrejoining events but signals of unresolved DNA damage

Marta Martín, Mariona Terradas, Laia Hernández, Anna Genescà

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

© 2014 Taylor & Francis Group, LLC. The presence of γH2AX foci on apparently intact mitotic chromosomes is controversial because they challenge the assumed relationship between γH2AX foci and DNA double-strand breaks (DSBs). In this work, we show that after irradiation during interphase, a variety of γH2AX foci are scored in mitotic cells. Surprisingly, approximately 80% of the γH2AX foci spread over apparently undamaged chromatin at Terminal or Interstitial positions and they can display variable sizes, thus being classified as Small, Medium and Big foci. Chromosome and chromatid breaks that reach mitosis are spotted with Big (60%) and Medium (30%) Terminal γH2AX foci, but very rarely are they signaled with Small γH2AX foci. To evaluate if Interstitial γH2AX foci might be signatures of misrejoining, an mFISH analysis was performed on the same slides. The results show that Interstitial γH2AX foci lying on apparently intact chromatin do not mark sites of misrejoining, and that misrejoined events were never signaled by a γH2AX foci during mitosis. Finally, when analyzing the presence of other DNA-damage response (DDR) factors we found that all γH2AX foci - regardless their coincidence with a visible break - always colocalized with MRE11, but not with 53BP1. This pattern suggests that these γH2AX foci may be hallmarks of both microscopically visible and invisible DNA damage, in which an active, although incomplete or halted DDR is taking place.
Original languageEnglish
Pages (from-to)3026-3036
JournalCell Cycle
Volume13
Issue number19
DOIs
Publication statusPublished - 1 Oct 2014

Keywords

  • Illegitimate joining
  • M phase
  • Misrepair
  • MRE11
  • Residual DSBs
  • Telomeric foci
  • γH2AX

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