TY - JOUR
T1 - β5 integrin is the major contributor to the α v integrin-mediated blockade of HIV-1 replication
AU - Ballana, Ester
AU - Pauls, Eduardo
AU - Clotet, Bonaventura
AU - Perron-Sierra, Françoise
AU - Tucker, Gordon C.
AU - Esté, José A.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Monocytes and macrophages are targets of HIV-1 infection and play critical roles in multiple aspects of viral pathogenesis. During the differentiation of monocytes to macrophages, adhesion molecules such as integrins are upregulated; therefore, they provide signals that control the process and subsequently may render macrophages more susceptible to HIV-1 infection. Previous work demonstrated that blocking α v-containing integrins triggered a signal transduction pathway leading to the inhibition of NF-κB-dependent HIV-1 transcription. In this paper, we show the influence of the different α v-coupled β integrins in HIV-1 replication in macrophages. Inhibition of β integrins, either by specific mAbs, small arginine-glycine-aspartic acid (RGD) mimetic compounds, or RNA interference, showed that integrin β 5 was the major contributor to the integrin-mediated blockade of HIV-1 replication. Importantly, such inhibition did not induce changes in cell adhesion to the substrate. In conclusion, our results reveal a significant role of the integrin dimmer α vβ 5 in HIV-1 infection of macrophages. Copyright©2010 by The American Association of Immunologists, Inc.
AB - Monocytes and macrophages are targets of HIV-1 infection and play critical roles in multiple aspects of viral pathogenesis. During the differentiation of monocytes to macrophages, adhesion molecules such as integrins are upregulated; therefore, they provide signals that control the process and subsequently may render macrophages more susceptible to HIV-1 infection. Previous work demonstrated that blocking α v-containing integrins triggered a signal transduction pathway leading to the inhibition of NF-κB-dependent HIV-1 transcription. In this paper, we show the influence of the different α v-coupled β integrins in HIV-1 replication in macrophages. Inhibition of β integrins, either by specific mAbs, small arginine-glycine-aspartic acid (RGD) mimetic compounds, or RNA interference, showed that integrin β 5 was the major contributor to the integrin-mediated blockade of HIV-1 replication. Importantly, such inhibition did not induce changes in cell adhesion to the substrate. In conclusion, our results reveal a significant role of the integrin dimmer α vβ 5 in HIV-1 infection of macrophages. Copyright©2010 by The American Association of Immunologists, Inc.
U2 - 10.4049/jimmunol.1002693
DO - 10.4049/jimmunol.1002693
M3 - Article
VL - 186
SP - 464
EP - 470
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 1
ER -