β-Cell function abnormalities in islets from an adult subject with nesidioblastosis and autoantibodies against the islet cells

Ignacio Conget, Yolanda Sarri, Nuria Somoza, Marta Vives, Anna Novials, Aurelio Ariza, Josefa Fernández-Alvarez, Ricardo Pujol-Borrell, Ramon Gomis

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Human islets from an adult subject with nesidioblastosis were isolated and used to perform in vitro studies. Isolated nesidioblastotic islets showed an increased basal rate of insulin secretion (nesidioblastotic islets, 81.3 ± 6.4 vs. control islets, from cadaveric organ normal donors, 10.2 ± 0.9 μU/islet/90 min) without any further release with increasing glucose concentration. In addition, islets isolated from the pancreas with nesidioblastosis contained more insulin than control islets, 1,547.0 ± 128.7 vs. 935.0 ± 51.7 μU/islet, and the levels of insulin mRNA were also higher than those measured in controls. Interestingly, the serum of the subject with nesidioblastosis contained autoantibodies that stained brightly and selectively a population of islet cells whose distribution coincided with that expected of α cells. In summary, pancreatic islets in nesidioblastosis display β-cell functional abnormalities. Moreover, the finding of antibodies against islet cells is a common feature in the serum of nesidioblastotic subjects; nevertheless, their pathological significance warrants further investigation.
Original languageEnglish
Pages (from-to)71-75
JournalPancreas
Volume14
DOIs
Publication statusPublished - 1 Jan 1997

Keywords

  • Antiislet antibodies
  • Insulin content
  • Insulin response
  • Nesidioblastosis
  • Pancreatic islets

Fingerprint Dive into the research topics of 'β-Cell function abnormalities in islets from an adult subject with nesidioblastosis and autoantibodies against the islet cells'. Together they form a unique fingerprint.

Cite this