ß-Adrenergic receptor signaling increases NAADP and cADPR levels in the heart

Alexander M. Lewis; Parvinder K. Aley; Ali Roomi; Justyn M. Thomas; Roser Masgrau; Clive Garnham; Katherine Shipman; Claire Paramore; Duncan Bloor-Young; Luke E.L. Sanders; Derek A. Terrar; Antony Galione; Grant C. Churchill

doi:https://doi.org/10.1016/j.bbrc.2012.09.054

ß-Adrenergic receptor signaling increases NAADP and cADPR levels in the heart

Alexander M. Lewis, Parvinder K. Aley, Ali Roomi, Justyn M. Thomas, Roser Masgrau, Clive Garnham, Katherine Shipman, Claire Paramore, Duncan Bloor-Young, Luke E.L. Sanders, Derek A. Terrar, Antony Galione, Grant C. Churchill

Research output: Contribution to journal › Article › Research › peer-review

18 Citations (Scopus)

Abstract

Evidence suggests that ß-Adrenergic receptor signaling increases heart rate and force through not just cyclic AMP but also the Ca 2+-releasing second messengers NAADP (nicotinic acid adenine dinucleotide phosphate) and cADPR (cyclic ADP-ribose). Nevertheless, proof of the physiological relevance of these messengers requires direct measurements of their levels in response to receptor stimulation. Here we report that in intact Langendorff-perfused hearts ß-adrenergic stimulation increased both messengers, with NAADP being transient and cADPR being sustained. Both NAADP and cADPR have physiological and therefore pathological relevance by providing alternative drug targets in the ß-adrenergic receptor signaling pathway. © 2012 Elsevier Inc.

Original language	English
Pages (from-to)	326-329
Journal	Biochemical and Biophysical Research Communications
Volume	427
DOIs	https://doi.org/10.1016/j.bbrc.2012.09.054
Publication status	Published - 19 Oct 2012

Keywords

Calcium
Cyclic ADP-ribose
Heart
Nicotinic acid adenine dinucleotide phosphate
ß-Adrenergic receptor

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Lewis, A. M., Aley, P. K., Roomi, A., Thomas, J. M., Masgrau, R., Garnham, C., Shipman, K., Paramore, C., Bloor-Young, D., Sanders, L. E. L., Terrar, D. A., Galione, A., & Churchill, G. C. (2012). ß-Adrenergic receptor signaling increases NAADP and cADPR levels in the heart. Biochemical and Biophysical Research Communications, 427, 326-329. https://doi.org/10.1016/j.bbrc.2012.09.054

Lewis, Alexander M. ; Aley, Parvinder K. ; Roomi, Ali ; Thomas, Justyn M. ; Masgrau, Roser ; Garnham, Clive ; Shipman, Katherine ; Paramore, Claire ; Bloor-Young, Duncan ; Sanders, Luke E.L. ; Terrar, Derek A. ; Galione, Antony ; Churchill, Grant C. / ß-Adrenergic receptor signaling increases NAADP and cADPR levels in the heart. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 427. pp. 326-329.

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title = "{\ss}-Adrenergic receptor signaling increases NAADP and cADPR levels in the heart",

abstract = "Evidence suggests that {\ss}-Adrenergic receptor signaling increases heart rate and force through not just cyclic AMP but also the Ca 2+-releasing second messengers NAADP (nicotinic acid adenine dinucleotide phosphate) and cADPR (cyclic ADP-ribose). Nevertheless, proof of the physiological relevance of these messengers requires direct measurements of their levels in response to receptor stimulation. Here we report that in intact Langendorff-perfused hearts {\ss}-adrenergic stimulation increased both messengers, with NAADP being transient and cADPR being sustained. Both NAADP and cADPR have physiological and therefore pathological relevance by providing alternative drug targets in the {\ss}-adrenergic receptor signaling pathway. {\textcopyright} 2012 Elsevier Inc.",

keywords = "Calcium, Cyclic ADP-ribose, Heart, Nicotinic acid adenine dinucleotide phosphate, {\ss}-Adrenergic receptor",

author = "Lewis, {Alexander M.} and Aley, {Parvinder K.} and Ali Roomi and Thomas, {Justyn M.} and Roser Masgrau and Clive Garnham and Katherine Shipman and Claire Paramore and Duncan Bloor-Young and Sanders, {Luke E.L.} and Terrar, {Derek A.} and Antony Galione and Churchill, {Grant C.}",

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ß-Adrenergic receptor signaling increases NAADP and cADPR levels in the heart. / Lewis, Alexander M.; Aley, Parvinder K.; Roomi, Ali; Thomas, Justyn M.; Masgrau, Roser; Garnham, Clive; Shipman, Katherine; Paramore, Claire; Bloor-Young, Duncan; Sanders, Luke E.L.; Terrar, Derek A.; Galione, Antony; Churchill, Grant C.

In: Biochemical and Biophysical Research Communications, Vol. 427, 19.10.2012, p. 326-329.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Lewis, Alexander M.

AU - Aley, Parvinder K.

AU - Roomi, Ali

AU - Thomas, Justyn M.

AU - Masgrau, Roser

AU - Garnham, Clive

AU - Shipman, Katherine

AU - Paramore, Claire

AU - Bloor-Young, Duncan

AU - Sanders, Luke E.L.

AU - Terrar, Derek A.

AU - Galione, Antony

AU - Churchill, Grant C.

PY - 2012/10/19

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AB - Evidence suggests that ß-Adrenergic receptor signaling increases heart rate and force through not just cyclic AMP but also the Ca 2+-releasing second messengers NAADP (nicotinic acid adenine dinucleotide phosphate) and cADPR (cyclic ADP-ribose). Nevertheless, proof of the physiological relevance of these messengers requires direct measurements of their levels in response to receptor stimulation. Here we report that in intact Langendorff-perfused hearts ß-adrenergic stimulation increased both messengers, with NAADP being transient and cADPR being sustained. Both NAADP and cADPR have physiological and therefore pathological relevance by providing alternative drug targets in the ß-adrenergic receptor signaling pathway. © 2012 Elsevier Inc.

KW - Calcium

KW - Cyclic ADP-ribose

KW - Heart

KW - Nicotinic acid adenine dinucleotide phosphate

KW - ß-Adrenergic receptor

U2 - https://doi.org/10.1016/j.bbrc.2012.09.054

DO - https://doi.org/10.1016/j.bbrc.2012.09.054

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