The aim of the present project is to continue thea studies carried out in our Group about the mechanisms involved in the mutagenic process using different genetic systems of D. melanogaster. This have been specially developed to analyse somatic mutations and mitotic recombination named SMAR (Somatic Mutation And Recombination). The free radicals produced by the cellular metabolism itself, or as conssequence of the action of certain physical or chemical agents, can produce DNA double strand breakages that stimulate the mitotic recombination, or if they qre not correctly repaired, give place to samatic mutations. On one hand, we want to analyse the mutagenic-antimutagènic interactions in those cases where the genotoxic agents produces oxidative damage; and, on the other hand, find out the basic molecular mechanisms involved in the genetic systems used for their study, as well as the importance that the DNA repair mechanisms have on the genomic instability. Therefore, the precise objectives of this study can be grouped in to three general objectives: 1) the study of the mutagenic-antimutagenic interactions in the case of compounds that produce oxidative damage, 2) The analysis of the mechanisms that are involved in the genetic SMART systems, and 3) the study of the DNA repair mechanisms in Drosophila.
|Effective start/end date||19/12/00 → 19/12/03|