Reconeixement de ligands epitelials i restrcció per CD1 en autoimmunitat. Paper de les subpoblacions en lifocits T no convencionals.

Project Details


Study objective: Analysis of T cells of non conventional phenotype and of their possible role in autoimmunity. Design: Phenotype, functional TCR analysis and study of the recognition of CD1 and of epithelial ligands by cells expanded from the infiltrates of three different autoimmune diseases. Organ specific Graves' disease and Hashimoto's thyroiditis; Systemic Sjögren syndrome; and autoimmunity induced by external factors (gluten): celiac disease. In all thre model epithelial cells are targets. Previous studies in thyroid autoimmunity, we have isolated \gamma\delta cells with epithelial specificity as well as NKT-like cells (double negative CD3+). In both, no classical MHC restriction was observed. This led us to study the recognition of alternative presenting molecules such as CD1. Preliminary data suggest that CD1 is expressed in several epithelia and that CD1-reactive T cells may be involved in the regulation both positive and negative, of the \i in situ\i0 autoimmune response. Our objective is to analyse these cells in detain in the thyroid samples and to confirm the role of these in celiac disease. Measurements: cytotoxicity and cytokine production in front of CD1 and epithelial cells. Analysis of TCR in isolated cells by RT-PCR and sequencing.
Effective start/end date2/03/9931/12/01


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