Plants systems differ from animal in a number of properties. Between them, and related to the control of cell division and differentiation, are the special characteristics of meristems and the property of totipotency of many plant somatic cells. The role of the protein kinase p34 cdc32 in the cell cycle control is being elucidated during the last few years, but almost nothing is know about another related protein kinase, the casein kinase II. However, there are many experimental data that establish a structural and functional relationship between them. The main goal of this project is to study the role of the casein kinase II in the control of cellular proliferation and differenciation by analyzing its expression during the cell cycle. Using Arabidopsis cell suspension cultures, we will measure the following parameters: a) mRNA and protein levels; b) autophosphorylation; c) enzymatic act; d) subcellular compartimentation of the protein. Furthermore, we will overexpress the protein in plant protoplats and make constructs to express mRNA antistene in the same system, in order to observe the phenotypes caused by alterations in CKII levels. Finally, we will determine the CKII levels. Finally, we will determine the CKII exprexxion in differentiated tissue \i in situ\i0 hybridization and immunolocalization in the adult plant.
|Effective start/end date||30/09/94 → 30/09/97|