Modulació de la reactivitat glial i la funció de la medul·la espinal de la rata després de lesions traumàtiques i fotoquímica

  • Navarro Acebes, Xavier (Principal Investigator)
  • Ceballos Afán de Rivera, Dolores (Researcher on contract)
  • Garcia Alias, Guillermo (Researcher on contract)
  • Verdú Navarro, Enrique (Researcher on contract)
  • Cuadras Mas, Jordi (Investigator)
  • Forés Viñeta, Joaquim (Investigator)
  • Nieto Sampedro, Manuel (Investigator)

Project Details

Description

We propose to study the changes in function and glial reactivity that follow spinal cord lesiones, and the modulation of both by olfactory bulb ensheathing cell (OBEC) transplants combined with neuroprotective agents. Contunsive or photochemical spinal cord lesions will be inflicted in adult rats, immediately following which the animal will be treated as follows: i) a suspensión of selected cells will be transplanted in the injured cord; ii) various neuroprotective agents (methylprednisolone, FK506, memantine); iii) combined cell transplant and pharmacological agent. Glial reactivity and its modulation by the various agents will be assesed by immunohistochemical determination of inflamatory cytokines, astrocyte and microglia activation and neuronal degeneration and death. Comparative gene expression in embryonic, neonatal and adult OBECs, examined by means of gene chips (microarrays), will be correlated to its modulatory efficiency. Lesion-derived changes in spinal cord function will be measured by kinematic analysis of locomotion and electrophysiological determination of spinal reflex circuits. The data will allow us to establish whether OBEC transplants, with or without neuroprotective agents, prevent secondary neuronal death and glial reactivity following CNS lesions. We want to examine whether growth stimulating microenvironment is thus generated, promoting regenerative axon growth in the surviving neurons and correcting the functional deficits arising from the lesion
StatusFinished
Effective start/end date1/12/021/12/05

Funding

  • Ministerio de Ciencia y Tecnología: €87,400.00

Fingerprint Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.