Genomics and Protenics have become hot fields in Biology. Description of the whole set of genes from an organism permits a new type of analysis, being its final goal the description of a complex biological system at a quantitative molecular level. Howewer, to accomplish this goal more basic knowledge of the interrelations and biological functions of their genes and proteins is necessary. The analysis and redesign of a simple and autoreplicative minimal cell, previously checked by Nature as is micoplasma, can contribute to this knowledge. With 480 genes (22% of them with unknown functiion), 38 metabolic pathways, low level of gene regulation and a simple and typical membrane, is a perfect model to redesign its genome and metabolism, and anallyse its proteome to get insight into what constitutes a living system. This is teh fundamental objetive of teh project. To fulfill this objetive, computacional genomic snalysis will be combined with be combined with metabolic and cellular engineering. Some of the redesing to be performed are: the system of electronic transfer to oxygen; designing a pathway analog to theKrebs cycle; redesigning the liposacchaaride metabolism (cholesterol and glycolipids), in addition, structure/function studies of key enzymes from these metabolic pathways will be also performed. Still, it will be tried to disclose the putative biological function of the high and unexpected number of genes, many of them essential, with unknown function.
|Effective start/end date||28/12/01 → 27/12/03|