Genomics and Proteins have become hot fields in Biology. -description of the whole set of genes from an organism permits a new type of analysis, being its final goal the description of a complex biological system at a quantitative molecular level. However, to accomplish this goal more basic knowledge of the interrelations and biological functions of their genes and proteins is necessary. The analysis and redesign of a simple anc autoreplicative minimal cell, previously checked by Nature, as is myciplasma,can contribute to this knowledge. With 480 genews (22% of them with unknown function), 38 metabolic pathways, low level of gene regulation and a simple and typical membrane, is a perfect model to redesign its genome and metabolism, and analyse its proteome to get insight into what constitutes a living system. This is the fundamental objective of the project. To fulfill this objective, computational genomic analysis will be combined with metabolic and cellular engineering. Some of the redesigns to b pervormed are: the system of electronic transfer to oxygen; designing a pathway analog to the Krebs cycle; redesigning the liposaccharide metabolism (cholesteros anc glycolipids). In addition, structure/function studies of key enzymes from these metabolic pathways will performed too. On the other hand, the proteome and cell-map proteomics will be characterized. Some putative biotechnological applications of this microorganism will be developed, for example, expression in its membrane of heterologous proteins with clinical interest or the improvement of the kinetics of growth for vaccine production. Still, it will be tried to disclose the putative biological function of the high and unexpected number of genes, many of them essential, with unknown function.
|Effective start/end date||28/12/00 → 28/12/01|
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