"Alpha sub1" adrenergic receptors stimulate phosphoinositide phospholipase C (PLC) but it has not been clarified whether the activation of PLC is direct (receptor-G protein-PLC) or results from Ca"super2+" entry. In order to identify transduction mechanisms coupled to these receptors we propose to compare the efficacies of noradrenaline, muscarinic agonists and Ca"super2+" ionophores stimulating the accumulation of inositol phosphates and CDP-diacilglycerol (intermediate metabolite in the phosphatidylinositol resynthesis) in rat brain cortex under different experimental conditions since these responses are modulated by Ca"super2+" in opposite directions. We also plan to set up a model of experimental gangliosidosis and to study its effects on noradrenaline responses. The patterns of accumulation of the different isomers of inositol phosphates will be compared and the possible role of the
|Effective start/end date||22/06/92 → 22/06/95|
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