We have carried out research on the mechanism of formation of nucleosome core particles. Our results have allowed us to suggest dynamic models that could be useful in understanding the involvement of DNA-histone complexes in the replication and transcription of eukariotik organisms. In this project we are planning to extend these studies to the whole nucleosome (i.e., including histone H1) and higher order chromatin structures. Furthermore, in this part of the project, in order to analyze chromatin fragments of relatively high molecular weight, we will try to combine two electrophoretic methods: gel retardation and pulsed-field gel electrophoresis. From the methodological point of view, the research of procedures for the analysis of DNA-protein complexes using pulsed-field gel electrophoresis could be very important in the future development of this new and complex technique that at pres
|Effective start/end date||2/08/90 → 2/08/92|
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