The aim of this Project is to analyse the chromosome position effect in the interphasic nucleus in phylogeneticaly related species, related to the probability of interchromosome evolutive rearrangements; and to characterize from the molecular point of view the region affected by the rearrangement. For this purpose we will determine the territory occupied for each chromosome in the interphasic nucleus in different primate cell lines. Our interest is to see if the position of homologous chromosomes is conserved I the evolution, and if there is a relationship between the proximity and the capacity of interchromosomical rearrangement during speciation processes. Three primate cell lines from different species will be used, Zoo-FISH techniques will be used to localise the different chromosomes in the nucleus. Observations will be made in a Laser Confocal Microscope to obtain tridimensional reconstructions of the nucleus. Different parameters will be obtained and analysed foe each nucleus, results will be analysed separately for each species and the three species will be compared. Data of different behaviour of chromosomes during evolution in relation to interchromosomal rearrangements will be considered in the study. Finally, the breakpoints implicated in chromosome rearrangements will be localised by comparative screening of BAC and cosmid libraries from human and Saimiri sciureus, and the scanning of the clones of interest by the design of pairs of primers from PCR reactions and the final sequencing of a fragment of 3 Kb that contains the breakpoint
|Effective start/end date||13/12/04 → 13/12/07|
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