The Saccharomyces cereviae genome project finished in 1996, revealing a potential of approcimately 6200 Open Reagind Frames, with approximately 3000without experimentally detemined function. Since years is a good model organism to understand the function of the mammalian genes, a great international effort is being made to determine the function of all mammalian genes. a great international effort is being made to determine the dunction of all their genes. By using a consensus sequence belonging to the medium-chain dehydrogenases/reductases (MDR), with Zn in their active-site, we have identified four ORFs that presumably belong to the MDR superfamily in yeast. We have found the function for one of them,that was a (2R,3R)-2,3-butanediol dehydrogenase. although this enzyme is not essential for yeast, a deleted strain attained a lesser cell density than the wild-type strain when growing with 2-3-butanediol as the sole carbon cource. One of the main objectives from the present project is to ascertain the biological function for the remaining MDR. To this end we will characterize their phenotypes. We will, finally study their subcellular localization. A second main objective of this project is to study the S-nitrosoglutathione reductase activity of ADH class III from yeast and human.Given the fact, that rat ADH class III has also a N-nitrosoglutathione reductase activity, the enzyme could contribute to the nitric oxide signalling pathway and also to the protection of cysteine thiol groups, and indirectally to the tyrosine hydroxyl groups, and also nuclear DNA against nitrosilation.
|Effective start/end date||19/12/00 → 19/12/03|
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