The main objective of this study is to assess the protective role that alcohol dehydrogenase (ADH) may offer against oxidative stress during chronic ethanol consumption. Three groups of samples will be analyzed: samples obtained from fifty chronic alcoholic patients and from fifty healthy subjects, as a control; samples obtained from rats fed chronically with ethanol, and samples from hepatoma cell culture. Two parameters indicating oxidative stress will be determined: lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) and antioxidant status (alfa-tocopherol and glutathione). The results obtained will be correlated with ADH genotypes in humans and determination of ADH activity levels in rat and cell culture samples.
|Effective start/end date||1/01/94 → 31/12/96|
- Fondo de Investigación Sanitaria: €55,894.13