Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols (ROBOX)

Project Details


Oxygen functionalities are key functional groups in many of today’s chemicals and materials. The efficient introduction of oxygen-functionalities into raw materials are key chemical transformations in bulk and fine chemicals. Innovative bio-catalytic oxidation routes using molecular oxygen (from air) under benign and mild (pH) conditions such as ambient temperature and pressure can greatly improve the sustainability and economics of processes, but were so far mainly been applied in the pharma segments. In this segment, the enzyme-catalyzed step often represents the highest added value and the high price of the end-product (> €1000/kg) justifies less than optimal enzyme production and limitations in its catalytic efficiency.

In order to achieve the widening of industrial application of enzymatic bio-oxidation processes to also larger volume but lower price chemical markets, ROBOX will demonstrate the techno-economic viability of bio-transformations of four types of robust oxidative enzymes: P450 monooxygenases (P450s), Baeyer-Villiger MonoOxygenase (BVMOs), Alcohol DeHydrogenase (ADH) and Alcohol OXidase (AOX) for which target reactions have already been validated on lab-scale in pharma, nutrition, fine & specialty chemicals and materials applications. ROBOX will demonstrate 11 target reactions on large scale for these markets in order to prepare them for scale up to commercial-scale plants.

ROBOX is industry-driven with 2 major industrial players and 6 SME’s. It will assess the potential of technologies applied to become platform technologies technologies (multi-parameter screening systems, computational methodologies, “plug bug” expression systems) for broad replication throughout the chemical industry. The markets addressed within ROBOX represent a joint volume of over 6.000 ktons/year. The introduction of bio-oxidation processes is expected to bring substantial reductions in cost (up to -50%), energy use (-60%), chemicals (-16%) and GHG-emissions (-50%).
Effective start/end date1/04/151/04/19

Collaborative partners

  • Maastricht University
  • Austrian Centre of Industrial Biotechnology (ACIB)
  • CheamStream BVBA
  • Technical University of Denmark (Danmarks Tekniske Universiteit)
  • Dyadic Nederland B.V.
  • Eidgenössische Technische Hochschule Zürich
  • evocatal GmbH
  • Fraunhofer Society for the Advancement of Applied Research
  • Genencor International RV
  • Lentikat's a.s
  • PNO Consultants BV
  • Rijksuniversiteit Groningen
  • Rheinisch Westfälischen Technische Hochshule Aachen
  • Graz University of Technology
  • Universitat Autònoma de Barcelona (UAB) (lead)
  • University of Manchester
  • University of Pavia (UNIPV)


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