Estudis moleculars sobre la ribonucleasa A: catàlisi, interacció enzim-substrat i plegament

  • Cuchillo Foix, Claudi Miquel (Principal Investigator)
  • Bravo Camisón, María Isabel (Scholar)
  • Mahtat, Said (Scholar)
  • Boix Borras, Ester (Investigator)
  • Coll Constans, Maria Gràcia (Investigator)
  • Nogués Bara, Maria Victoria (Investigator)
  • Ribó Panosa, Marc (Investigator)
  • Vilanova Brugués, Maria E. (Investigator)

Project Details

Description

Bovine pancreatic ribonuclease A is a well known enzyme which has been used as a general model for structure-function relationship studies of nucleases. In the present project, studies about the catalytic process, enzyme-substrate binding and folding are covered. The functional role of non-catalytic phosphate binding subsites adjacent to the catalytic site will be analysed. Likewise, studies about the mobility of amino acid residues of the catalytic and non-catalytic sites will be carried out. The protein folding studies will be focused on the contribution of hydrophobic forces of the C-terminal amino acid residues on the global stability of the protein. Protein engineering by site direct mutagenesis will be applied to these studies.
StatusFinished
Effective start/end date8/07/948/07/97

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