Estudis dels mecanismes de regulació mitjançant fosforil·lació de proteïnes en llevat

Project Details


Reversible protein phosphorylation is a major mechanism of protein modification and play san important role in all life forms by regulating important and different cellular functions. Protein kinases (PK) and phosphatases (PP), the enzymes that catalyse phosphorylation and desphosphorylation reactions, are responsible of changes in enzymatic activity, cellular localisation, or binding partners of the substrates. The data generated after different sequencing projects indicates that more than 2% of the genomes encode for PK and PP and has made evident that we are far from having a complete view of the complex cell signalling pathways. Therefore it is obvious that new strategies are necessaries in order to achieve a faster development of this field. I plan to use the combination of the powerful genomics and proteomics technology together with genetics and biochemistry to study the signalling events, using yeast cells as eukaryotic model, in which protein phosphorylation reactions are involved. Generation and analysis of expression patters induced by deletion of non-essential PK and PP will be performed. Samples will be collected from cells growing not only in standard conditions but also subjected to a set of different stress conditions. Clustering those genes that induce similar expression profiles (i.e encoding for proteins working in the same pathways) will allow us to assign new or additional functions to the analysed PK and PP. Furthermore, different proteomic techniques will be used to both, identify new regulatory mechanisms of PK and PP activities, and to look for new substrates at global scale of those proteins
Effective start/end date13/12/0413/12/07


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