One of the main problems to get a successful orthotopic transplantation of the larynx is related with the reinnervation of it. Intimately related with this problem, there are several aspects of the neuroanatomy and neurophysiology of the larynx which in spite of the clinical and experimental studies are without resolutio, as it is the explanation about variable position of the vocal cords after a laryngeal paralysis. This variability has been considered due to different factors: organisation of the neural fibres of the recurrent nerve activity of the crycothyroid muscle, residual innervation or reinnervation of the laryngeal muscles and anastomosis between the laryngeal nerves. The objective of this project is to obtain an experimental model which permit us evaluate the role play the laryngeal anastomosis andreproduce situations of reinnervation (with and withour reparation) related with different types of laryngeal paralysis by means of a battery of neurophysiological test. The study will be made by the microdissection of 25 human larynges in correlation with a experimental model (Sprague-Dawley rat). At the end of the neurophysiological test, by means of histological methods, we will quantify axonal regeneration and the degree of target tissue reinnervated. In summary, a detailed morphological and functional study of the nerve supply of the larynx in order to obtain an experimental model may become an effective instrument to be employed in surgey with the aim of improve the technics of neural reparation after a laryngeal paralysis and orthotopics transplantation of the larynx.
|Effective start/end date||2/03/99 → 31/12/01|
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