Estudi del patró de reorganització morfofuncional del nucli ambigu com a conseqüència d'una secció del nervi recurrent en un model experimental de laringe de rata adulta i neonatal

Project Details

Description

The possibility to doing orthotopic transplants of larynx or functional reinervation human in laryngeal palsy need the knowledge of the morphological events not only a peripheral level (muscles and nerves) but also a central level (morpho-functional reorganization) of the cellular groups by a lesion. In the laryngeal palsy due to lesion of the recurrent nerve the position of the vocal folds is quite variable. Re-anatomosis of the stumps of the recurrent nerve has been done to avoid the above-mentioned problems. However, this technique has not been useful from the functional point of view due to the non-selective reinervation (synkinesis). Other techniques has been apply, selective reinervation with neuromuscular pedicles from other nerves and muscles. It has also shown not satisfactory functional results. There are only two studies that have made a correlation between lesion of the recurrent nerve and reorganization of the somatopia in the NA, considering that the reinervation it is a non-selective phenomenon. In another experimental model with similar nerves (facial nerve palsy), it has been reported a selective reinnervation in young animals and also a faint disorganization of its motor nucleus in adult animals.
Based on the experience of the group in a laryngeal model of laryngeal palsy, the aim of this project it is to stablish a correlation, after lesion of the recurrent nerve, between the reinervation process that take place in the muscles and nerves with the somatopia of the NA in young and adult samples. For getting this goal we will use different fluorescent traces place in the adductor and abductor muscles of Sprague-Dawley rats (section). The functional results will be evaluated by laryngoscopy and the anatomical results of the muscles by inmunofluorescence (PGP), morphometry of the nerves and 3D reconstructions of the NA will be make in the different experimental groups.
The results of this project will permit to stablish and accurate anatomi
StatusFinished
Effective start/end date29/11/0328/11/06

Funding

  • Instituto de Salud "Carlos III": €69,345.00

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