Estudi de les alteracions genètiques al diagnòstic i seguiment i anàlisi del quimerisme a leucemies infantils mitjançant tècniques citogenètiques.

  • Coll Sandiumenge, M. Dolors (Principal Investigator)
  • Caballin Fernandez, Rosa (Assessor)
  • Armengol Rosell, Gemma (Scholar)
  • Alvarez Cobo, Yolanda (Investigator)
  • Gaitán Alcalde, Susana (Scholar)
  • Ortega Blanco, Margarita (Fundación del Vall (Scholar)
  • Bastida Vila, Pilar (Investigator)

Project Details

Description

\ul Objectives\ul0 . To stablish the diagnosis in patients with hematological malignancies by cytogenetics, FISH, comparative genomic hybridization and Southern blot. 2. To carry out the follow-up in order to detect the minimal residual disease (MRD) in patients under transplantion by the above-mentioned techniques. 3. To analyce the chimerism by FISH and PCR for DNA polymorphism and SRY gene. \ul Design\ul0 . Bone marrow and/or blood extraction will be carried out per patient. The following studies will be done: 1) Identification the chromosome abnormality at diagnosis. 2) Detection of MRD in autologous and allorgenic hamatopoietic progenitor transplantation (HPT), every 3 months during 2 years. 3) Analysis of chimerism in alogenic HPT every 3 months during 2 years. \ul Setting\ul0 . Patients under HPT from all Spain will be included because "Unidad de Transplante de la Vall d'Hebron" is a reference center. \ul Patients\ul0 . 80-100 patients for diagnosis and 40-50 for the follow-up MRD and chimerism study. \ul Interventions\ul0 . Bone marrow and blood harvesting at diagnosis and post-HPT will be performed in Hospital materno-infantil Vall d'Hebron. Samples will be sent to the Department of the UAB. \ul Measurements\ul0 . Morphological blood and bone marrow studies; erycthrocyte markers, lymphocyte subpopulations. Genetic rearrangements. Numerical and structural chromosome aberrations.
StatusFinished
Effective start/end date10/09/9831/12/00

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