Estudi de les alteracions genètiques al diagnòstic i seguiment i anàlisi del quimerisme a leucemies infantils mitjançant tècniques citogenètiques.

Project Details


\ul Objectives\ul0 .
To stablish the diagnosis in patients with hematological malignancies by cytogenetics, FISH, comparative genomic hybridization and Southern blot. 2. To carry out the follow-up in order to detect the minimal residual disease (MRD) in patients under transplantion by the above-mentioned techniques. 3. To analyce the chimerism by FISH and PCR for DNA polymorphism and SRY gene.
\ul Design\ul0 .
Bone marrow and/or blood extraction will be carried out per patient. The following studies will be done: 1) Identification the chromosome abnormality at diagnosis. 2) Detection of MRD in autologous and allorgenic hamatopoietic progenitor transplantation (HPT), every 3 months during 2 years. 3) Analysis of chimerism in alogenic HPT every 3 months during 2 years.
\ul Setting\ul0 .
Patients under HPT from all Spain will be included because "Unidad de Transplante de la Vall d'Hebron" is a reference center.
\ul Patients\ul0 .
80-100 patients for diagnosis and 40-50 for the follow-up MRD and chimerism study.
\ul Interventions\ul0 .
Bone marrow and blood harvesting at diagnosis and post-HPT will be performed in Hospital materno-infantil Vall d'Hebron. Samples will be sent to the Department of the UAB.
\ul Measurements\ul0 .
Morphological blood and bone marrow studies; erycthrocyte markers, lymphocyte subpopulations. Genetic rearrangements. Numerical and structural chromosome aberrations.
Effective start/end date10/09/9831/12/00


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