Project Details
Description
\ul Objectives\ul0 .
To stablish the diagnosis in patients with hematological malignancies by cytogenetics, FISH, comparative genomic hybridization and Southern blot. 2. To carry out the follow-up in order to detect the minimal residual disease (MRD) in patients under transplantion by the above-mentioned techniques. 3. To analyce the chimerism by FISH and PCR for DNA polymorphism and SRY gene.
\ul Design\ul0 .
Bone marrow and/or blood extraction will be carried out per patient. The following studies will be done: 1) Identification the chromosome abnormality at diagnosis. 2) Detection of MRD in autologous and allorgenic hamatopoietic progenitor transplantation (HPT), every 3 months during 2 years. 3) Analysis of chimerism in alogenic HPT every 3 months during 2 years.
\ul Setting\ul0 .
Patients under HPT from all Spain will be included because "Unidad de Transplante de la Vall d'Hebron" is a reference center.
\ul Patients\ul0 .
80-100 patients for diagnosis and 40-50 for the follow-up MRD and chimerism study.
\ul Interventions\ul0 .
Bone marrow and blood harvesting at diagnosis and post-HPT will be performed in Hospital materno-infantil Vall d'Hebron. Samples will be sent to the Department of the UAB.
\ul Measurements\ul0 .
Morphological blood and bone marrow studies; erycthrocyte markers, lymphocyte subpopulations. Genetic rearrangements. Numerical and structural chromosome aberrations.
To stablish the diagnosis in patients with hematological malignancies by cytogenetics, FISH, comparative genomic hybridization and Southern blot. 2. To carry out the follow-up in order to detect the minimal residual disease (MRD) in patients under transplantion by the above-mentioned techniques. 3. To analyce the chimerism by FISH and PCR for DNA polymorphism and SRY gene.
\ul Design\ul0 .
Bone marrow and/or blood extraction will be carried out per patient. The following studies will be done: 1) Identification the chromosome abnormality at diagnosis. 2) Detection of MRD in autologous and allorgenic hamatopoietic progenitor transplantation (HPT), every 3 months during 2 years. 3) Analysis of chimerism in alogenic HPT every 3 months during 2 years.
\ul Setting\ul0 .
Patients under HPT from all Spain will be included because "Unidad de Transplante de la Vall d'Hebron" is a reference center.
\ul Patients\ul0 .
80-100 patients for diagnosis and 40-50 for the follow-up MRD and chimerism study.
\ul Interventions\ul0 .
Bone marrow and blood harvesting at diagnosis and post-HPT will be performed in Hospital materno-infantil Vall d'Hebron. Samples will be sent to the Department of the UAB.
\ul Measurements\ul0 .
Morphological blood and bone marrow studies; erycthrocyte markers, lymphocyte subpopulations. Genetic rearrangements. Numerical and structural chromosome aberrations.
| Status | Finished |
|---|---|
| Effective start/end date | 10/09/98 → 31/12/00 |
Funding
- Fondo de Investigación Sanitaria
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