Aneuploidy is the most common class of chromosome abnormality in humans. It is the most important cause of mental retardation and it has been associated with cancer progression and spontaneous abortions in humans. It is unfortunate that relatively little research has been accomplished to detect the ability of ionizing radiations to induce aneuploidy compared to other genotoxic endpoints (e.g. gene mutations and chromosome breakage). The present proposal concerns the investigation of the induction and persistence of aneuploidy after in vivo radiation of male mice. This work is suggested to be carried out both in somatic and germ cells by application of in situ hybridization methodologies. Due to the lack of mouse centromeric commercial DNA probes, these will be obtained in our own lab by microdissection of mouse chromosomes, amplification and labelling of DNA followed by their use as DNA probes in FISH protocols. The specific objectives of the present proposal are: 1. Obtention ofpericentromeric DNA probes for three mouse chromosomes. 2. Analysis of anaphase lag and non-disjunction induced in mouse spleen lymphocytes after irradiation of mice. The analysis will be carried out in interphase nuclei of binucleated splenocytes. 3. Analysis of the chromos nal content of micronus' ;i by microdissection and reverse painting on normal mouse chromosome metaphases. 4. Analysis of induction of aneuploidy in mouse spermatozoa after irradiation of male mice.
|Effective start/end date||22/09/00 → 22/09/03|
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