Estudi de la diferenciació cardiogénica de cèl·lules mare i desenvolupament de matrius biocompatibles colonitzades per precursors cardíacs per a la regeneració del miocardi

  • Cairo Badillo, Jordi Joan (Principal Investigator)
  • Juanola Journé, Sandra (Researcher on contract)
  • Lecina Veciana, Martí (Researcher on contract)
  • Sarro Casanovas, Enric (Researcher on contract)
  • Solà Ferrando, Carles (Investigator)

    Project Details


    Hypothesis: The human injured myocardium does not have ability to regenerate. Cell therapy based on bone marrow-derived autologous progenitor cell mobilization or the exogenous administration of these progenitor cells may be an efficient alternative to conventional medical therapy.
    1.To study the mobilization, homing and functional implantation of endothelial progenitor cells in patients with and without dilated cardiomyopathy.
    2.Induction of differentiation of bone marrow-derived mesenchymal stem cells into cardiomyocytes and characterization of their structural and electrophysiological properties.
    3.To develop, with cooperation of myocardiogenic and endothelial progenitors, viable and functionally active myocardial tissue within 3-D polymers and scars from infracted hearts: cardiac tissue engineering.
    Working plan: extraction and in vitro culture of bone marrow-derived mesenchymal stem cells and circulating endothelial progenitors. Characterization of in vitro vasculogenesis from endothelial progenitors using ad hoc developed microbioreactors. Chemical stimulation, cytokines and growth factors, co-culture and metabolic and physical modulation necessary to transdifferentiate mesenchymal stem cells to mature cardiomyocytes (structurally and electrically).
    Infarct-derived scars will be cultured in vitro in combination with progenitor endothelial and mesenchymal cells to transdifferentiate the fibrotic tissue into myocardium-like tissue Finally, we will examine the ability to these stem cells to colonize 2-D and 3-D collagen structures in order to create tissue matrices that resemble myocardium, which may have a therapeutic application to regenerate injured hearts
    Effective start/end date13/12/0413/12/07


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