Estudi de la diferenciació cardiogénica de cèl·lules mare i desenvolupament de matrius biocompatibles colonitzades per precursors cardíacs per a la regeneració del miocardi

  • Cairo Badillo, Jordi Joan (Principal Investigator)
  • Juanola Journé, Sandra (Researcher on contract)
  • Lecina Veciana, Martí (Researcher on contract)
  • Sarro Casanovas, Enric (Researcher on contract)
  • Solà Ferrando, Carles (Investigator)

    Project Details

    Description

    Hypothesis: The human injured myocardium does not have ability to regenerate. Cell therapy based on bone marrow-derived autologous progenitor cell mobilization or the exogenous administration of these progenitor cells may be an efficient alternative to conventional medical therapy.
    Objectives:
    1.To study the mobilization, homing and functional implantation of endothelial progenitor cells in patients with and without dilated cardiomyopathy.
    2.Induction of differentiation of bone marrow-derived mesenchymal stem cells into cardiomyocytes and characterization of their structural and electrophysiological properties.
    3.To develop, with cooperation of myocardiogenic and endothelial progenitors, viable and functionally active myocardial tissue within 3-D polymers and scars from infracted hearts: cardiac tissue engineering.
    Working plan: extraction and in vitro culture of bone marrow-derived mesenchymal stem cells and circulating endothelial progenitors. Characterization of in vitro vasculogenesis from endothelial progenitors using ad hoc developed microbioreactors. Chemical stimulation, cytokines and growth factors, co-culture and metabolic and physical modulation necessary to transdifferentiate mesenchymal stem cells to mature cardiomyocytes (structurally and electrically).
    Infarct-derived scars will be cultured in vitro in combination with progenitor endothelial and mesenchymal cells to transdifferentiate the fibrotic tissue into myocardium-like tissue Finally, we will examine the ability to these stem cells to colonize 2-D and 3-D collagen structures in order to create tissue matrices that resemble myocardium, which may have a therapeutic application to regenerate injured hearts
    StatusFinished
    Effective start/end date13/12/0413/12/07

    Funding

    • Ministerio de Educación y Ciencia: €69,000.00

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