Desenvolupament de proteïnes quimèriques recombinants portadores d'epítops vírics. Aplicació al disseny i la millora d'antígens.

Project Details

Description

The use of viral peptides correctly located and presented at the surface of carrier recombinant proteins can increase the specificity of such antigens as well as their reactivity with monoclonal antibodies and polyclonal sera. In this work, we propose the development of chimeric "beta"-galactosidase and p9 proteins carrying unique and multiple insertions of the antigenic site A from foot-and-mouth disease virus, and the study of their antigenic and immunogenic behaviour when produced in "E. coli". We are interested in specific insertion sites or combinations of them in which the recombinant peptide could be recognized more efficiently than the synthetic peptide reproducing the inserted stretch, since chemically synthetic peptides are often used as tools in diagnosis of viral infections. These products will be studied and their antigenic features improved by protein engineering. We also p
StatusFinished
Effective start/end date1/07/951/07/98

Funding

  • Comisión Interministerial de Ciencia y Tecnología (CICYT): €112,749.86

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