Cell division is at the basics of all morphogenic events and a complete undesrstanding of the molecular mechanisms involved in its control is essential for developmental studies and has a wide range of biotechnological applications. In the present project we propose to study the role of two enzymes, protein kinase CK2 and glutathione-dependent formaldehyde dehydrogenase, in several aspects of cell-cycle control. Protein kinase CK2 is a kinase whose activity increases rapidly after stimulation of cell division. It shows a cell-cycle dependent regularion, with peaks of activity at G1/S and M phases. The regulation is attained at a post-translational level, mainly, by allosteric activation of the enzyme, post-transcriptional control of protein concentration and subcellular targeting, among others. Our goal is a deep understanding of the different mechanisms controlling CK2 activity, to establish the basic determinants necessary for its function. This knowledge will provide insights to understand the role of CK2 in cell-cycle control, in specifically related to the accomplishment of G2/M checkpoint. The glutathone-dependent formaldehyde dehydrogenase is the main enzyme for formaldehyde metabolism in living cells. Formadehyde is a side-product in oxodative stress and very toxic for the cells. The formaldehyde dehydrogenase has a double cellular localization, in the nucleus and in the cytoplasm. The study of cellk-cycle regularion of formaldehyde dehydrogenase might establish links between the transduction pathways used by both cell division cycle and several types of stress. Biotechnological applications in bioremediation will also be studied.
|Effective start/end date||19/12/00 → 19/12/03|