DNA probes for the identification of clinical isolates of Salmonella and Shigella will be constructed. The probes will consist in fragments of genus-specific insertion sequences (the Salmonella-specific element IS 200 and the Shigella specific IS 630). Two methods of the identification will employed: 1) DNA hybridisation; 2) polymerase chain reaction (PCR). In a attempt to optimise nonradiactive labelling, dimeric probes will be constructed; if labelled whit the ECL system, these dimmers are expected to allow amplification of the hybridisation signal. For PCR identification, oligonucleotides 20-30 bp long, each identical to the sequence of an unambiguous region of the corresponding element, will be used. The accuracy of the identification method will be estimated from surveys on distribution of the elements Is 200 and IS 630 among a large number of Salmonella and Shigella isolates, provided from several hospitals
|Effective start/end date||1/04/91 → 31/03/92|
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