This project aims at contributing to the development and integrated application of bioprocess engineering and metabolic engineering tools allowing the optimization of recombinant protein production processes in the yeast Pichia pastoris, as well as the industrial application of the obtained recombinant products in biotransformations of interest for the pharmaceutical and veterinary industry. Our efforts will be focused on the minimization of the major limitations detected in P. pastoris-based production systems, using a multidisciplinary approach integrating genetic, metabolic/physiologic and operational strategies, with the overall objective increase the bioprocess productivity and reproducibility, as well as recombinant product quality up to a pilot plant scale (bioreactor capacity 60 litres). Two major protein classes will be produced in the framework of this project. Microbial (fungal and yeast) lipases, particularly from Rhizopus oryzae and Candida rugosa, for their application in the resolution of racemic mixtures of products of interest for the pharmaceutical and food industry. Animal hormones, particularly the equine chorionic gonadotropin (eCG) as a model for glycoprotein complex, as well a of interest for the veterinary industry, as it stimulates estrogens and progesterone production in pregnant mares. The novel production processes generated from the implementation of the new operational and metabolic engineering strategies will be compared with the currently available processes. Also, the produced recombinant products will be characterized and compared with their commercially available counterparts (currently obtained from native sources) and their functionality evaluated. Overall, all the integrative efforts here proposed has a clear applied objective, that is, to provide to industry with optimal strategies for recombinant protein production based on the P. pastoris system, leading to maximal process productivity and reproducibility(...)
|Effective start/end date||1/10/07 → 30/09/10|
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