We attempt to adequate the FISH chromosomal painting technique to human sperm chromosomes and to applicate this method to cytogenetic studies on carriers of chromosome rearrangements selected from thouse ascertained in Clinical Genetic departments (we intend to include criptic translocations). Sperm chromosome complements will be obtained by hamster oocyte-human sperm fusion to allow chromosomal identification. The sperm chromosome complements will be treated with FISH-painting techniques and with classical banding methods. After cytogenetic analysis we are going to determine the meiotic behaviour in each chromosomal rearrangement, the frecuency of spermatozoa with chromosomal abnormalities, the existence of the interchromosomal effects, if differencies exist between the two identifying methods, and the empiric risk.
|Effective start/end date||8/07/94 → 8/07/97|
- Dirección General de Investigación Científica y Técnica (DGICYT)