The principal aim of this work is to isolate and characterize the "lexA" gene of several gram-negative bacteria to compare both its structure and its product. The species to study will be the "Acinetobacter calcoaceticus", the "Aeromonas caviae", the "Agrobacterium tumefaciens", the "Erwinia carotovora", the "Proteus mirabilis", the "Pseudomonas aeruginosa", the "Pseudomonas putida", the "Pseudomonas syringae", the "Rhizobiummeliloti" and the "Salmonella typhimurium". The cloning of these genes will be performed by using a system developed in our laboratory, and composed by the "pUA165" plasmid and the "UA4793" ("LexA(Def)SulA-HsdR"sup"-") strain of "E. Coli". This strategy allows us to directly isolate the "lexA"-like gene of any gram-negative bacteria. To achieve our objective, chromosomal libraries of all these bacterial species will be constructed. The"pUAB165" plasmid will be the cl
|Effective start/end date||3/07/92 → 3/07/95|
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