Description
Specifically, it contains:<br>
<ul><li>TEM images characterizing the Calu-3 barrier establishment. Air liquid Interface lung epithelial images specifying the different observed characteristics.</li>
<li>A SEM image of the PLA nanoparticles used in the study (PH_PLA).</li>
<li>Confocal images illustrating particle internalisation at different time points. 3D images of Calu-3 barriers untreated and treated to PLA-NPLs for 24 h and 2 weeks, respectively. Orthogonal views demonstrating the presence of PLA-NPLs inside the cell cytoplasm. 3D images with cross-sectional views demonstrating the presence of PLA-NPLs in the nuclear compartment. PLA-NPLs, cell nuclei, and ZO-1 junctions are seen in green, blue, and red,
respectively. </li>
<li>Confocal images of immunostaining of tight junctions after PLA treatment. Images of untreated, and 24 hours and 2 weeks treated Calu-3 barriers with 2.5 µg/cm2 PLA-NPLs. In blue the cell nuclei and in red the tight junction ZO-1.</li>
<li>Inverted light microscopy images of Calu-3 barriers stained with Alcian Blue and hematoxylin-eosin, highlighting mucus, cellular nuclei, and cytoplasm.</li></ul>
| Date made available | 12 Mar 2025 |
|---|---|
| Publisher | CORA.Repositori de Dades de Recerca |
Projects
- 2 Finished
-
DESARROLLO DE NAMS PARA LA EVALUACION DE LOS EFECTOS PARA LA SALUD DE CONTAMINANTES EMERGENTES PRESENTES EN LAS AGUAS RESIDUALES
Hernandez Bonilla, A. (Principal Investigator), Marcos Dauder, R. (Co-Investigador/a Principal), Alaraby Abdalaziz Mohamed, M. (Collaborator), Rubio Lorente, L. (Collaborator), Tarrio Fernandez, R. M. (Collaborator), Tavakolpournegari ., A. (Collaborator), Umbert Maestre, M. G. (Collaborator), Villacorta Villacorta, A. (Collaborator), Valiente Gil, C. (Collaborator), Barguilla Moreno, I. (Investigator), Rodriguez Trelles Astruga, F. J. (Investigator), Velazquez Henar, M. A. (Investigator), Xamena Lopez, N. (Investigator) & Banaei, G. (Collaborator)
1/09/21 → 31/08/24
Project: Research Projects and Other Grants
-
PLASTICHEAL: Innovative tools to study the impact and mode of action of micro and nanoplastics on human health: towards a knowledge base for risk assessment
Hernandez Bonilla, A. (Coordinator), Marcos Dauder, R. (Principal Investigator), Barbadilla Prados, A. (Investigator), Buffa Dunat, M. N. (Investigator), Casillas, S. (Investigator), Juan Godoy, B. (Investigator), Pastor Benito, S. (Investigator), Perez Gonzalez, G. (Investigator), Valiente Malmagro, M. (Investigator), Velazquez Henar, M. A. (Investigator), López Mesas, M. (Co-Investigador/a Principal), Villacorta Villacorta, A. (Investigator), Rubio Lorente, L. (Investigator), Garcia Rodriguez, A. (Investigator), Alaraby Abdalaziz Mohamed, M. (Investigator), Abass, D. (Investigator), Martín Pérez, J. (Predoctoral), Gutierrez Garcia, J. (Predoctoral), Morataya Reyes, H. M. (Predoctoral), Tavakolpournegari ., A. (Predoctoral), Banaei, G. (Predoctoral), Barguilla Moreno, I. (Investigator), Cervera Hurtado, M. (Project Manager), Arribas Arranz, J. (Collaborator) & Annangi ., B. (Collaborator)
1/04/21 → 31/03/25
Project: International research project
Research output
- 1 Article
-
Polylactic acid nanoplastics (PLA-NPLs) induce adverse effects on an in vitro model of the human lung epithelium: The Calu-3 air-liquid interface (ALI) barrier
García-Rodríguez, A., Gutiérrez, J., Villacorta, A., Arribas Arranz, J., Romero-Andrada, I., Lacoma, A., Marcos, R., Hernández, A. & Rubio, L., 15 Aug 2024, In: Journal of Hazardous Materials. 475, 16 p., 134900.Research output: Contribution to journal › Article › Research › peer-review
Open Access33 Link opens in a new tab Citations (Scopus)1 Downloads (Pure)
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