Replication data for: "Impact of adding oocyte-secreted factors BMP15 and GDF9 to the IVM medium of prepubertal goat oocytes on cumulus-oocyte communication, EGF receptor and embryo developmental competence"

Raw data of research article "Impact of adding oocyte-secreted factors BMP15 and GDF9 to the IVM medium of prepubertal goat oocytes on cumulus-oocyte communication, EGF receptor and embryo developmental competence". The aim of this study was to assess the effect of adding two of the main OSFs, the BMP15 and GDF9, to IVM medium of prepubertal goat oocytes. Cumulus-oocyte complexes (COCs) were in vitro matured in absence (control group) or presence of 100 ng/mL of BMP15, GDF9, or both. To determine cumulus-oocyte communication, transzonal projections (TZP) density at 0h, 6h, 12h and 24h of IVM were evaluated.
After IVM, the epidermal growth factor receptor (EGFR) expression in oocytes and their cumulus cells, cumulus expansion, mitochondrial activity and intracellular ROS and glutathione (GSH) levels were assessed. Finally, cleavage and blastocyst rates after parthenogenetic activation (PA) and IVF, and blastocyst quality by cell count were assessed.