Additional file 6: Figure S6. of Quaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs

Details of the relative position of Rluc and YFP in a receptor heterotetramer interacting with two G proteins. Computational-based model of Gs and Gi bound to the adenosine A1R-A2AR heterotetramer. Rluc and YFP fused to the N-terminal domain of the Gα-subunits point toward different positions in space (A), whereas Rluc and YFP fused to G γ -subunits are close (B). The color code of the proteins is depicted in the adjacent schematic representations (TM4 and TM5 of GPCR protomers are in light blue and gray, respectively). (TIF 6445 kb)