Additional file 2: Figure S2. of Quaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs

Graphical description of the stoichiometry of A1R-GFP, A2AR-mCherry or both A1-GFP and A2A-mCherry. The fluorescence intensity signal distribution (gray area) detected for more than 7000 independent observations is given for HEK-293T cells expressing A1-GFP (A), A2A-mCherry (D), or both A1-GFP and A2A-mCherry (B, E). The stoichiometry analysis was performed for A1-GFP (A, B) and A2A-mCherry (D, E). Curves approximately delineating the amount of monomers, dimers, trimers, and tetramers are displayed in green for A1-GFP (A, B) and in red for A2A-mCherry (D-E). The occurrence on the cell surface of monomers, dimers, trimers, and tetramers for A1-GFP (C) expressed alone (black bars) or in the presence of A2A-mCherry (blue bars) and for A2A-mCherry (F) expressed alone (black bars) or in the presence of A1-GFP (blue bars) was calculated by stoichiometry analysis from results shown in A, B, D, and E. (TIF 455 kb)