Structural modeling and functional characterization of mammalian cytosolic carboxypeptidases 2 and 3

Resum

Aquesta_x000D_ tesis_x000D_ s’emmarca_x000D_ en_x000D_ el_x000D_ camp_x000D_ de_x000D_ les_x000D_ carboxipeptidases_x000D_ citosòliques_x000D_ de_x000D_ mamífers,_x000D_ centrant-­‐se_x000D_ particularment_x000D_ en_x000D_ els_x000D_ membres_x000D_ 2_x000D_ i_x000D_ 3_x000D_ d’aquesta_x000D_ subfamília_x000D_ de_x000D_ metalocarboxipeptidases._x000D_ Aquest_x000D_ treball_x000D_ constitueix_x000D_ el_x000D_ primer_x000D_ estudi_x000D_ sobre_x000D_ la_x000D_ funció_x000D_ d’aquests_x000D_ enzims_x000D_ a_x000D_ través_x000D_ d’aproximacions_x000D_ in_x000D_ silico,_x000D_ in_x000D_ vitro_x000D_ i_x000D_ in_x000D_ vivo._x000D_ Aquesta_x000D_ tesis_x000D_ consta_x000D_ de_x000D_ les_x000D_ parts_x000D_ descrites_x000D_ a_x000D_ continuació._x000D_ El_x000D_ primer_x000D_ capítol_x000D_ introdueix_x000D_ el_x000D_ camp_x000D_ de_x000D_ les_x000D_ matalocarboxipeptidases,_x000D_ els_x000D_ microtúbuls,_x000D_ les_x000D_ modificacions_x000D_ post-­‐traduccionals_x000D_ de_x000D_ la_x000D_ tubulina_x000D_ i_x000D_ estableix_x000D_ els_x000D_ objectius_x000D_ de_x000D_ la_x000D_ tesi._x000D_ El_x000D_ segon_x000D_ capítol_x000D_ és_x000D_ una_x000D_ compilació_x000D_ dels_x000D_ procediments_x000D_ experimentals_x000D_ i_x000D_ materials_x000D_ utilitzats_x000D_ en_x000D_ el_x000D_ treball_x000D_ experimental._x000D_ El_x000D_ tercer_x000D_ capítol_x000D_ presenta_x000D_ la_x000D_ caracterització_x000D_ funcional_x000D_ de_x000D_ la_x000D_ carboxipeptidasa_x000D_ citosòlica_x000D_ 3_x000D_ humana._x000D_ Els_x000D_ resultats_x000D_ d’aquest_x000D_ estudi_x000D_ han_x000D_ estat_x000D_ la_x000D_ base_x000D_ pel_x000D_ quart_x000D_ capítol,_x000D_ on_x000D_ es_x000D_ descriu_x000D_ la_x000D_ funció_x000D_ de_x000D_ la_x000D_ CCP2._x000D_ Ambdues_x000D_ CCP2_x000D_ i_x000D_ CCP3_x000D_ isoformes_x000D_ de_x000D_ ratolí_x000D_ s’estudien_x000D_ mitjançant_x000D_ enginyeria_x000D_ de_x000D_ proteïnes_x000D_ i_x000D_ utilitzant_x000D_ els_x000D_ models_x000D_ de_x000D_ ratolí_x000D_ knockout_x000D_ de_x000D_ CCP2_x000D_ i_x000D_ CCP3._x000D_ Els_x000D_ capítols_x000D_ III_x000D_ i_x000D_ IV_x000D_ es_x000D_ subdivideixen_x000D_ en_x000D_ un_x000D_ resum_x000D_ del_x000D_ capítol,_x000D_ els_x000D_ resultats_x000D_ que_x000D_ descriuen_x000D_ el_x000D_ detall_x000D_ dels_x000D_ descobriments_x000D_ i_x000D_ una_x000D_ discussió._x000D_ A_x000D_ continuació_x000D_ es_x000D_ presenta_x000D_ una_x000D_ discussió_x000D_ general_x000D_ sobre_x000D_ CCP2_x000D_ i_x000D_ CCP3,_x000D_ el_x000D_ seu_x000D_ rol_x000D_ en_x000D_ la_x000D_ cèl·∙lula_x000D_ i_x000D_ en_x000D_ el_x000D_ context_x000D_ de_x000D_ la_x000D_ subfamília_x000D_ de_x000D_ carboxipeptidases_x000D_ que,_x000D_ en_x000D_ mamífers,_x000D_ consta_x000D_ de_x000D_ sis_x000D_ gens_x000D_ codificants_x000D_ per_x000D_ enzims_x000D_ amb_x000D_ activitat_x000D_ deglutamilasa._x000D_ La_x000D_ tesis_x000D_ finalitza_x000D_ amb_x000D_ un_x000D_ resum_x000D_ de_x000D_ les_x000D_ conclusions._x000D_ El_x000D_ capítol_x000D_ IV_x000D_ juntament_x000D_ amb_x000D_ els_x000D_ experiments_x000D_ de_x000D_ modelat_x000D_ estructural_x000D_ del_x000D_ capítol_x000D_ III_x000D_ són_x000D_ la_x000D_ base_x000D_ de_x000D_ la_x000D_ següent_x000D_ publicació_x000D_ científica:_x000D_ Tort_x000D_ O,_x000D_ Tanco_x000D_ S,_x000D_ Rocha_x000D_ C,_x000D_ Bièche_x000D_ I,_x000D_ Seixas_x000D_ C,_x000D_ Bosc_x000D_ C,_x000D_ Andrieux_x000D_ A,_x000D_ Moutin_x000D_ MJ,_x000D_ Avilés_x000D_ FX,_x000D_ Lorenzo_x000D_ J,_x000D_ Janke_x000D_ C._x000D_ The_x000D_ cytosolic_x000D_ carboxypeptidases_x000D_ CCP2_x000D_ and_x000D_ CCP3_x000D_ catalyze_x000D_ posttranslational_x000D_ removal_x000D_ of_x000D_ acidic_x000D_ amino_x000D_ acids._x000D_ MBoC._x000D_ doi:10.1091/mbc.E14-­‐06-­‐1072

Data del Ajut	15 d’oct. 2014
Idioma original	No s'ha definit/desconegut
Supervisor	Francesc Xavier Aviles Puigvert (Director/a), Sebastian Martin Tanco . (Director/a) & Julia Lorenzo Rivera (Director/a)