TY - JOUR
T1 - Widespread circulation of flaviviruses in horses and birds in northeastern spain (Catalonia) between 2010 and 2019
AU - Napp, Sebastian
AU - Llorente, Francisco
AU - Beck, Cécile
AU - Jose‐cunilleras, Eduard
AU - Soler, Mercè
AU - Pailler‐garcía, Lola
AU - Amaral, Rayane
AU - Aguilera‐sepúlveda, Pilar
AU - Pifarré, Maria
AU - Molina‐lópez, Rafael
AU - Obón, Elena
AU - Nicolás, Olga
AU - Lecollinet, Sylvie
AU - Jiménez‐clavero, Miguel Ángel
AU - Busquets, Núria
N1 - Funding Information:
IRTA?CReSA and CISA?INIA (CSIC) are funded by PID2020?116768RR?C21/C22 projects. The work was funded by the Departament d?Acci? Clim?tica, Alimentaci? i Agenda Rural. IRTA is supported by the CERCA Programme/Generalitat de Catalunya. The National Institute for Agricultural and Food Research and Technology (CISA, INIA?CSIC) was also funded by E?RTA2015?00002?CO2?01 project.
Funding Information:
Funding: IRTA‐CReSA and CISA‐INIA (CSIC) are funded by PID2020‐116768RR‐C21/C22 projects. The work was funded by the Departament d’Acció Climàtica, Alimentació i Agenda Rural. IRTA is supported by the CERCA Programme/Generalitat de Catalunya. The National Institute for Agricultural and Food Re‐ search and Technology (CISA, INIA‐CSIC) was also funded by E‐RTA2015‐00002‐CO2‐01 project.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consist-ently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, high-lighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV over-lapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick‐borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework.
AB - The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consist-ently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, high-lighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV over-lapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick‐borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework.
KW - Bagaza virus
KW - Flaviviruses
KW - Spain
KW - Tick‐borne encephalitis virus
KW - Usutu virus
KW - West Nile virus
UR - http://www.scopus.com/inward/record.url?scp=85120702147&partnerID=8YFLogxK
U2 - 10.3390/v13122404
DO - 10.3390/v13122404
M3 - Article
C2 - 34960673
AN - SCOPUS:85120702147
SN - 1999-4915
VL - 13
JO - Viruses
JF - Viruses
IS - 12
M1 - 2404
ER -
