The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila

Ricard Marcos; Erico R. Carmona

doi:10.1007/978-1-4939-9646-9_19

The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila

Ricard Marcos, Erico R. Carmona

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© 2019, Springer Science+Business Media, LLC, part of Springer Nature. In spite of its pioneer use in detecting mutational processes, Drosophila still plays an important role in those studies aiming to detect and quantify the induction of DNA damage. Here we describe two assays, one detecting primary damage (the Comet assay) and the other detecting somatic mutation and recombination effects (wing-spot test). It is important to emphasize that somatic recombination is a key event in cancer development and no assays exist at present to detect and quantify somatic recombination processes, other than the spot tests developed in Drosophila.

Idioma original	English
Títol de la publicació	Methods in Molecular Biology
Pàgines	337-348
Nombre de pàgines	12
Volum	2031
ISBN (electrònic)	1940-6029
DOIs	https://doi.org/10.1007/978-1-4939-9646-9_19
Estat de la publicació	Publicada - 1 de gen. 2019

Sèrie de publicacions

Nom	Methods in Molecular Biology
Volum	2031

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10.1007/978-1-4939-9646-9_19

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http://www.mendeley.com/research/wingspot-comet-tests-useful-assays-detecting-genotoxicity-drosophila

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T1 - The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila

AU - Marcos, Ricard

AU - Carmona, Erico R.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - © 2019, Springer Science+Business Media, LLC, part of Springer Nature. In spite of its pioneer use in detecting mutational processes, Drosophila still plays an important role in those studies aiming to detect and quantify the induction of DNA damage. Here we describe two assays, one detecting primary damage (the Comet assay) and the other detecting somatic mutation and recombination effects (wing-spot test). It is important to emphasize that somatic recombination is a key event in cancer development and no assays exist at present to detect and quantify somatic recombination processes, other than the spot tests developed in Drosophila.

AB - © 2019, Springer Science+Business Media, LLC, part of Springer Nature. In spite of its pioneer use in detecting mutational processes, Drosophila still plays an important role in those studies aiming to detect and quantify the induction of DNA damage. Here we describe two assays, one detecting primary damage (the Comet assay) and the other detecting somatic mutation and recombination effects (wing-spot test). It is important to emphasize that somatic recombination is a key event in cancer development and no assays exist at present to detect and quantify somatic recombination processes, other than the spot tests developed in Drosophila.

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KW - Drosophila

KW - Genotoxicity

KW - Wing-spot test

UR - http://www.mendeley.com/research/wingspot-comet-tests-useful-assays-detecting-genotoxicity-drosophila

U2 - 10.1007/978-1-4939-9646-9_19

DO - 10.1007/978-1-4939-9646-9_19

M3 - Chapter

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SN - 1064-3745

VL - 2031

T3 - Methods in Molecular Biology

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EP - 348

BT - Methods in Molecular Biology

ER -

The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila

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