The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

J. Marquet, L. Rafecas, A. Cantos, M. Moreno, M. Cervera, F. Casado, M.V. Nogués, C.M. Cuchillo

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Resum

The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present. © 1993.
Idioma originalAnglès
Pàgines (de-a)1297-1306
RevistaTetrahedron
Volum49
Número6
DOIs
Estat de la publicacióPublicada - 5 de febr. 1993

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