TDP-43 Cytoplasmic Translocation in the Skin Fibroblasts of ALS Patients

Miguel A. Rubio, Mireia Herrando-Grabulosa, Roser Velasco, Israel Blasco, Monica Povedano, Xavier Navarro

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Resum

Diagnosis of ALS is based on clinical symptoms when motoneuron degeneration is significant. Therefore, new approaches for early diagnosis are needed. We aimed to assess if alterations in appearance and cellular localization of cutaneous TDP-43 may represent a biomarker for ALS. Skin biopsies from 64 subjects were analyzed: 44 ALS patients, 10 healthy controls (HC) and 10 neurological controls (NC) (Parkinson’s disease and multiple sclerosis). TDP-43 immunoreactivity in epidermis and dermis was analyzed, as well as the percentage of cells with TDP-43 cytoplasmic localization. We detected a higher amount of TDP-43 in epidermis (p < 0.001) and in both layers of dermis (p < 0.001), as well as a higher percentage of TDP-43 cytoplasmic positive cells (p < 0.001) in the ALS group compared to HC and NC groups. Dermal cells containing TDP-43 were fibroblasts as identified by co-labeling against vimentin. ROC analyses (AUC 0.867, p < 0.001; CI 95% 0.800–0.935) showed that detection of 24.1% cells with cytoplasmic TDP-43 positivity in the dermis had 85% sensitivity and 80% specificity for detecting ALS. We have identified significantly increased TDP-43 levels in epidermis and in the cytoplasm of dermal cells of ALS patients. Our findings provide support for the use of TDP-43 in skin biopsies as a potential biomarker.
Idioma originalEnglish
Número d’article209
Nombre de pàgines12
RevistaCells
Volum11
Número2
DOIs
Estat de la publicacióPublicada - 8 de gen. 2022

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  • CIBERNED_Patología neuromuscular clínica y experimental

    Navarro Acebes, X. (Investigador/a principal), Aguilera Avila, J. (Investigador/a), Arbat Plana, A. (Investigador/a), Bosch, A. (Investigador/a), Bruna Escuer, J. (Investigador/a), Candalija Iserte, A. M. (Investigador/a), Casas, C. (Investigador/a), Catala Solsona, J. (Investigador/a), Cobianchi , S. (Investigador/a), Coll Miro, M. (Investigador/a), Cuadras Mas, J. (Investigador/a), Del Valle Macia, J. (Investigador/a), Enriquez Barreto, L. (Investigador/a), Francos Quijorna, I. (Investigador/a), González Pérez, F. J. (Investigador/a), Hernández Martín, J. (Investigador/a), Herrando Grabulosa, M. (Investigador/a), Jaramillo Rodríguez, J. (Investigador/a), Lopez Vales, R. (Investigador/a), López Serrano, C. (Investigador/a), López Álvarez, V. M. (Investigador/a), Martínez Muriana, A. (Investigador/a), Modol Caballero, G. (Investigador/a), Morell Orduña, M. (Investigador/a), Oliva Muñoz, N. D. L. (Investigador/a), Rodriguez Alvarez, J. (Investigador/a), Romeo Guitart, D. (Investigador/a), Santos Rojas, D. H. (Investigador/a), Siedlecki Wullich, D. J. (Investigador/a), Torres Espín, A. (Investigador/a), Udina Bonet, E. (Investigador/a), Garcia Garcia, J. (Investigador/a), Lopez Santos, D. (Investigador/a) & Badia Puiggali, C. (Investigador/a)

    1/01/0831/12/30

    Projecte: Projectes i Ajuts a la Recerca

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