TY - JOUR
T1 - Synergistic role of nanoceria on the ability of tobacco smoke to induce carcinogenic hallmarks in lung epithelial cells
AU - Rubio, Laura
AU - Bach, Jordi
AU - Marcos, Ricard
AU - Hernández, Alba
PY - 2017/12/1
Y1 - 2017/12/1
N2 - © 2017 Future Medicine Ltd. Aim: Since controversial results have been obtained in studies dealing with nanoceria usefulness in biomedical applications, the transforming effects of long-term exposure to nanoceria in lung epithelial cells, alone or together with cigarette smoke condensate (CSC), were evaluated. Materials & methods: In vitro cell transformation techniques were used to study several hallmarks of carcinogenesis. Morphology, cell proliferation, gene expression, cell migration, anchorage-independent cell growth and cell secretome were analyzed. Results & conclusion: Data evidence no transforming ability of nanoceria, but support a synergistic role on CSC's transforming ability. A more noticeable spindle-like phenotype, increased proliferation rate, higher degree of differentiation status dysregulation, higher migration capacity, increased anchorage-independent cell growth and higher levels of MMP-9 and cell growth promoting capability, were observed. In addition, nanoceria co-exposure exacerbates the expression of FRA-1.
AB - © 2017 Future Medicine Ltd. Aim: Since controversial results have been obtained in studies dealing with nanoceria usefulness in biomedical applications, the transforming effects of long-term exposure to nanoceria in lung epithelial cells, alone or together with cigarette smoke condensate (CSC), were evaluated. Materials & methods: In vitro cell transformation techniques were used to study several hallmarks of carcinogenesis. Morphology, cell proliferation, gene expression, cell migration, anchorage-independent cell growth and cell secretome were analyzed. Results & conclusion: Data evidence no transforming ability of nanoceria, but support a synergistic role on CSC's transforming ability. A more noticeable spindle-like phenotype, increased proliferation rate, higher degree of differentiation status dysregulation, higher migration capacity, increased anchorage-independent cell growth and higher levels of MMP-9 and cell growth promoting capability, were observed. In addition, nanoceria co-exposure exacerbates the expression of FRA-1.
KW - CeO NP 2
KW - cell transformation
KW - cigarette smoke condensate co-exposure
KW - lung BEAS-2B cells
KW - nanoceria in vitro long-term exposure
U2 - 10.2217/nnm-2017-0205
DO - 10.2217/nnm-2017-0205
M3 - Article
SN - 1743-5889
VL - 12
SP - 2623
EP - 2635
JO - Nanomedicine
JF - Nanomedicine
IS - 23
ER -